FIG. 6.

LPA promotes cell survival of MSC over 4 weeks in vivo. (A) BLI reveals that more GM-preconditioned MSC-Luc survive over 28 days when delivered with 25 μM LPA (top right) compared with untreated cells (top left). OM+ preconditioned MSC-Luc (bottom left) have higher persistence over time, but also exhibit improved survival in LPA-containing gels (bottom right). (B) Quantification of luminescence over time shows that LPA significantly improves survival of GM MSC-Luc. OM+ conditioning alone had a protective effect over 28 days, regardless of LPA treatment. Data are normalized to luminescence from gels containing undifferentiated cells within each animal at each time point. (C) Representative CD90 staining from gels containing GM-conditioned MSC confirms poor cell survival at 28 days that is (D) improved with the addition of LPA. (E, F) MSC conditioned in OM+ persisted at 28 days both in the absence (E) or presence (F) of LPA. Magnification is 100×; scale bar represents 100 μm. **p<0.01 versus GM, ***p<0.001 versus GM (n=6). Color images available online at www.liebertpub.com/tea