FIG. 3.

Cytodex3™ bead fibrin gel sprouting assay. (A) Representative images of sprouts. Scale bar=100 μm. (B) Del-1 HUVEC formed more sprouts compared with eGFP HUVEC as early as day 1, and more sprouts were apparent for Del-1 HUVEC for at least 7 days in culture. (C) The length of the sprouts was also greater for Del-1 HUVEC compared with eGFP HUVEC for all time points. Sprouts were measured for 10 beads per condition. Graphs show average±SEM; N=3 (n=10); ANOVA with LSD post hoc and p<0.05 (*) considered significant. ANOVA, analysis of variance; HUVEC, human umbilical vein endothelial cells; SEM, standard error of the mean. Color images available online at www.liebertpub.com/tea