FIG. 5.

PDLSC effect on functional vessel formation. (A) Red fluorescent 70 kDa dextran was administered systemically to mice before implant removal on day 7. Following fixation and cryosectioning, tissues were stained for human CD31 (green) and imaged. Stromal cell identity appeared to modulate the retention of dextran within the vessel lumen, which indirectly suggests variable maturity of the vessels across conditions. Implants contain ECs only, EC+MSCs, or ECs+PDLSCs. Images are 10×magnification; scale bar=100 μm and 40×magnification of the same region; scale bar=20 μm. (B) Cryosections from day 7 containing red dextran were stained for human alpha smooth muscle actin (αSMA, green) and imaged at 10×magnification. Implants contain ECs only, ECs+MSCs, or ECs+PDLSCs. Color images available online at www.liebertpub.com/tea