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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Biochem J. 2014 Apr 15;459(2):417–425. doi: 10.1042/BJ20131037

Figure 1. Palmitoylation of wild-type and F508del CFTR.

Figure 1

(A) HEK293 cells transiently expressing CFTR were metabolically labeled with 3H-palmitic acid. Subsequent immunoprecipitation of CFTR indicated that both wild-type and F508del CFTR are palmitoylated (top panel). As a control, 2% of cell lysate was reserved for analysis by western blot prior to IP (lower panel). (B) Treatment with the palmitoylation inhibitor 2-BP (100 μM during labeling – 4 h) in stably transduced HeLa wild-type cells inhibits labeling of CFTR as indicated by 3H-palmitic acid (top panel). Cell lysate (2%) was studied by western blot prior to IP (lower panel).