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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Biochem J. 2014 Apr 15;459(2):417–425. doi: 10.1042/BJ20131037

Figure 3. Influence of palmitoylation on low temperature correction of F508del CFTR.

Figure 3

(A) Western blot of F508del CFTR grown at low temperature (27°C). Treatment with 2-BP led to diminished correction of F508del CFTR as indicated by the absence of band C. Halide efflux was measured (B) and quantified (C) by the SPQ fluorescence assay. Forskolin (20 μM) and genistein (50 μM) were added (solid arrow) to stimulate CFTR-dependent ion transport. The findings indicate a decrease in surface activity of low temperature corrected F508del CFTR when palmitoylation is inhibited by 2-BP. Dotted arrow = addition of dequenching buffer; double arrow = addition of quenching buffer. Results are shown as mean ± SEM and normalized to control cells. *P < 0.005; n = 3.