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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Biochem J. 2014 Apr 15;459(2):417–425. doi: 10.1042/BJ20131037

Figure 5. Immunofluorescence of HeLa F508del cells in the presence or absence of DHHC-7.

Figure 5

F508del CFTR was labeled in the absence (column I) and presence (columns II–IV) of overexpressed DHHC-7. Mutant CFTR and HA-tagged DHHC-7 were labeled with anti-NBD1 and anti-HA antibodies, respectively. Golgi (top panel IV) or ER (lower panel IV) compartments were detected with CellLight GFP (Molecular Probes). Immunofluorescence identified strong co-localization of F508del CFTR with DHHC-7 and Golgi markers. The data also describe increased levels of F508del CFTR following overexpression of DHHC-7.