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. 2014 Apr;27(2):371–418. doi: 10.1128/CMR.00122-13

TABLE 1.

PCR-based studies that have included PCR detection of E. moshkovskii rDNAh

Country Method No. of patients Sample selection criterion No. of samples tested % positive samples
 Reference(s)
E. histolyticaa E. dispara E. moshkovskiia E. moshkovskii only
Bangladesh Nested PCR 109 109 16 36 21 6 427
Ghana PCR-RLB 246 20 0 45 0 428
India Nested PCR-RFLPc 746 Microscopy positive 68 19 97 25 1 429
Thailand Multiplex PCR Microscopy positive 30 13 20 0 0 430
Iran Nested PCRd 1,037 Microscopy positive 88 0 100 1 1 431
India Nested PCR-RFLPd 1,720 Microscopy positive/culture positive 202 30 79 16 1 432
India Nested multiplex PCR 1,720 Microscopy positive/culture positive 202 35 84 18 0 433
Turkey Nested PCR-RFLPd 100 Diarrhea cases 100 23c NA 2 NA 434
Australia Nested PCRd 1,246 MSM/microscopy positive 54 5 65 31 15 44
Australia Nested PCRd 5,921 Microscopy positive 110 5 57 50 24 435, 436
Tunisia Nested PCRd Microscopy positive 27 0 89 7 0 437
India Nested multiplex PCRe Microscopy positive 202 35 85 18 NA 438
Tanzania Nested PCRd 136 HIV positive 136 4c 5 13 12 439
622 Controls 0 0 0 0
Thailand Multiplex real-time (FRET) PCR Microscopy positive 33 6 85 1 0 61
India Nested multiplex PCRe 246 Microscopy positive 49 12 29 8 NA 440
NAi PCR-pyrosequencing NA Microscopy positive/E. histolytica-E. dispar PCR positive 102 17 86 0 0 64
Iran PCRf 3,825 Microscopy positive 58 3 93 5 2 441
Brazil PCR-sequencing Microscopy positive/culture positive 29 14 66 3b 3b 63
Ecuador PCR-RLBg 674 Microscopy positive 101 0 8 0 0 442
Pakistan Nested multiplex PCRe 129 Diarrhea cases 129 9 19 19 NA 45
151 Controls 151 1 27 4 NA
Malaysia PCRf 500 500 6 17 3 1 46, 443–445
Malaysia Nested multiplex PCRf 426 426 9 4 1 1 446
a

Including mixed infections.

b

DNA isolated from culture.

c

Antigen detection only.

d

PCR primers are described in reference 427.

e

PCR primers are described in reference 433.

f

PCR primers are described in reference 430.

g

PCR primers are described in reference 428.

h

Abbreviations: RLB, reverse line blot; RFLP, restriction fragment length polymorphism; FRET, fluorescence resonance energy transfer; MSM, men who have sex with men; NA, not available.

i

Samples from the indicated study were from patients that were tested in Sweden, Denmark, and The Netherlands.