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. 2014 Apr;27(2):371–418. doi: 10.1128/CMR.00122-13

TABLE 5.

PCR-based assays for detection and molecular characterization of Cyclosporaf

Species Amplification method Detection method Target Sample type(s) Reference
Cyclospora, Eimeria Nested PCR Gel electrophoresis SSU rDNA Purified oocysts 464
Cyclospora, Eimeria Nested PCRa Gel electrophoresis SSU rDNA Stool 465
Cyclospora, Eimeria Nested PCR-RFLPa Gel electrophoresis SSU rDNA Raspberries 466
Cyclospora, Eimeria Nested PCRa Oligonucleotide ligation SSU rDNA Controls 467
Cyclospora, Eimeria Nested PCRa Sequencing SSU rDNA Stool 468
Cyclospora PCR Gel electrophoresis ITS1 188
Cyclospora Real-time PCR Hydrolysis probe SSU rDNA Controls 190
Cyclospora Real-time PCR Hydrolysis probe SSU rDNA Stool 191
Cyclospora, Eimeria Nested PCR-RFLP Gel electrophoresis SSU rDNA Water 469
C. cayetanensis, C. colobi, and Eimeria Multiplex nested PCRb Gel electrophoresis SSU rDNA Stool 470
Cyclospora, Eimeria Nested PCRa Gel electrophoresis SSU rDNA Sputum 471
Cyclospora, Eimeria Nested PCRa Gel electrophoresis SSU rDNA Stool 472
Cyclospora Real-time PCRe Hydrolysis probe SSU rDNA Stool 473
C. cayetanensis, C. colobi, and Eimeria Multiplex Nested PCRd Gel electrophoresis SSU rDNA Stool 194
Cyclospora, Eimeria Nested PCR-RFLPa Gel electrophoresis SSU rDNA Stool 192
C. cayetanensis PCR Gel electrophoresis ITS2 Spiked samples 474
Cyclospora PCRc Gel electrophoresis ITS1 Stool 189
Nested PCR Gel electrophoresis SSU rDNA Controls 450
Cyclospora, Cryptosporidium, Toxoplasma, and Sarcocystis Real-time PCR SYBR green MCA SSU rDNA Control samples 219
C. cayetanensis, C. colobi, and Eimeria Multiplex PCRd Luminex SSU rDNA Stool 193
C. cayetanensis, C. colobi, and Eimeria Multiplex nested PCRd Gel electrophoresis SSU rDNA Stool 184
Cyclospora Nested PCR Sequencing SSU rDNA Stool 475
Cyclospora Nested PCR Sequencing HSP70 Stool 195
C. cayetanensis, C. colobi, and Eimeria Nested PCRd Electrophoresis SSU rDNA Raspberry, basil, pesto 476
C. cayetanensis, C. cercopitheci Real-time PCR Hydrolysis probe SSU rDNA Raspberry, basil, pesto
C. cayetanensis Real-time PCR Hydrolysis probe HSP70 Raspberry, basil, pesto
a

PCR primers are described in reference 464.

b

First-round PCR primers are described in reference 464.

c

PCR primers are described in reference 188.

d

PCR primers are described in reference 470.

e

PCR primers are described in reference 190.

f

Abbreviations: SSU, small subunit; ITS, internal transcribed spacer; HSP, heat shock protein; RFLP, restriction fragment length polymorphism; MCA, melt curve analysis.