FIG 6.

Comparison of inflammations induced by PA adjuvanted with Alhydrogel versus Advax and murabutide combination adjuvant. (A and B) Groups of mice (n = 4) were immunized s.c. with a single 50-ng dose of PA adjuvanted with Alhydrogel (0.035 mg) or Advax (1 mg) plus murabutide (MB) (0.1 mg). Two days postimmunization, mice were injected with Prosense 750 FAST and then imaged using an IVIS imaging system. Representative images are shown for mice that received PA with Alhydrogel (A) or Advax plus murabutide (B). (C) The mean total fluorescence intensities over the injection site were quantitated using ImageJ, demonstrating levels of Prosense 750 cleavage for mice receiving PA vaccine with Alhydrogel that were significantly higher than those seen with the mice receiving Advax plus murabutide (P = 0.001).