Mucosal IgA responses were assessed by ELISA. Spleens, bursas of Fabricius, thymuses, harderian glands, lungs, tracheas, livers, bile, duodena, jejuna, ilea, ceca, and rectums were obtained from the vaccinated group. IgA responses in immune (A), digestive (B), and respiratory (C) organs were tested by DPV antigen-capture ELISA.