FIG 2.

Survival rates, body weights, and pulmonary viral titers of infected BALB/c mice. Three days before a viral challenge, mice received an intraperitoneal injection of imiquimod (50 μg) and PANENZA vaccine (3 μg of HA) in combination (VCI day −3; ⧫), vaccine alone (vaccine day −3; *), imiquimod alone (imiquimod day −3; ▲), or PBS (PBS day −3; □). On the day of infection, mouse-adapted A(H1N1)pdm09 virus (10 times the mouse LD₅₀) was inoculated intranasally. Survival (A) and body weight (B) were monitored for 14 days. n = 20 to 30 per group. Results are mean data from five experiments. *, P < 0.05; **, P < 0.01 (compared with the VCI day −3 group). (C) Lung samples were taken at the indicated times after virus infection and homogenized for pulmonary virus titration by quantitative real-time RT-PCR. n = 9 for the PBS group, and n = 8 for all of the other groups. *, P < 0.05; **, P < 0.01. Error bars indicate standard deviations.