FIG 5.

Comparison of miniaturized ELISPOT assay and cell-ELISA. Two healthy donors were tested with a restricted antigen panel to include proteins (TT, PPD, CMV lysate) and peptides (CMV pp65 peptide mix). The ELISPOT assay SFU counts in the left panels are referred to as 10 × 10³ PBMC for the two 384- and 1,536-well formats in order to make results of the two formats comparable. Significantly high (P < 0.01) SFU counts in the smaller format indicated by an asterisk were seen in one case only, but a consistent trend for higher efficiency of the 1,536-well format was evident. Cell-ELISA results, given as ng/ml IFN-γ concentration, were not corrected for plate format, as described in legend to Fig. 3.