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Complementation of pyrF and hisB mutations. Growth assays of strains UBOCC-3107 (WT) (●), UBOCC-3256 (ΔpyrF) (■), and UBOCC-3262 (ΔpyrF TERMP_00006::pUFH-2) (▲) (A) and of strains UBOCC-3107 (WT) (●), UBOCC-3260 (ΔpyrF-ΔhisB) (■), and UBOCC-3265 (ΔpyrF ΔhisB TERMP_00004::pUHH) (▲) (B) were carried out at 85°C in TAA medium without (A) or with (B) uracil. These growth experiments were performed at 0.1 MPa.
