TABLE 1.
List of strains and plasmids used in this study
Strain or plasmid | Description |
---|---|
Strains | |
NCC 533 | Lactobacillus johnsonii strain from the Nestec Culture Collection |
NCC 9333 | Δpox (38) |
NCC 9334 | ΔLJ_1826 (predicted to encode lactate oxidase; Lox) |
NCC 9337 | ΔLJ_1254 ΔLJ_1255 (predicted to encode NADH oxidase; Nox) |
NCC 9359 | ΔLJ_0548 ΔLJ_0549 (predicted to encode NADH flavin reductase) |
Plasmids | |
pDP749 | Temperature-sensitive, allele exchange plasmid for L. johnsonii NCC 533 (39) |
pDP889 | pDP749 construct for lox (LJ_1826) deletion |
pDP902 | pDP749 construct for nox (LJ_1254 and LJ_1255) deletion |
pDP1010 | pDP749 construct for LJ_0548 and LJ_0549 deletion |
pDP794 | pNZ124-based expression plasmid with LJ_0045 promoter and LJ_1125 terminator |
pDP1016 | pDP794 with LJ_0548 expression plasmid |
pDP1017 | pDP794 with LJ_0549 expression plasmid |
pDP1019 | pDP794 with LJ_0548 and LJ_0549 expression plasmid |