. 2014 Apr;80(7):2254–2260. doi: 10.1128/AEM.04070-13

TABLE 2.

Prokaryotic diversity indices calculated based on a cutoff of 97% similarity of 16S rRNA gene sequences and 5,652 reads per sample^a

Cellar age (yr)	Chao1	Observed OTU	Shannon index
1	403.2 ± 72.1^ac	247.2 ± 18.0^ac	3.26 ± 0.55^a
10	335.1 ± 33.1^bc	221.2 ± 17.3^a	4.70 ± 0.74^b
25	457.1 ± 62.0^a	287.5 ± 19.2^b	5.61 ± 0.16^c
50	413.3 ± 69.5^ac	275.0 ± 33.8^bc	5.50 ± 0.30^c

All data are presented as means ± standard deviations (n = 5). Values with different letters in a column mean significant differences at P < 0.05 as determined by ANOVA.