TABLE 3.
Biofilm formation in a microtiter plate assay and on glass and stainless steel coupons and hydrophobicity of a subset of 12 ovine E. coli strains at different temperatures
| Isolate | Serotype | eae type | Median OD595 in microtiter plate assay (n = 3) at temp (°C) of: |
Mean scorea at indicated temp (°C) on: |
Mean hydrophobicity (%) ± SD (n = 3) at temp (°C) of: |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Glass coupons |
Steel coupons |
|||||||||||||
| 12 | 20 | 37 | 12 | 20 | 37 | 12 | 20 | 37 | 12 | 20 | 37 | |||
| 2006-22-1242-54-2 | O103:H2 | Negativeb | 2.194 | 1.886 | 0.479 | 1 | 3 | 2 | 3 | 3 | 1 | 51 ± 19 | 52 ± 16 | 93 ± 2 |
| 2006-22-1246-51-2 | O103:H2 | Negative | 1.048 | 1.744 | 1.364 | 1 | 3 | 1 | 3 | 3 | 2 | 47 ± 3 | 66 ± 5 | 90 ± 1 |
| 902006-22-1271-55-2 | O103:H2 | Negative | 1.089 | 1.722 | 0.644 | 1 | 3 | 1 | 3 | 3 | 2 | 62 ± 1 | 57 ± 7 | 93 ± 0 |
| 2006-22-1153-55-2 | O103:H2 | eaeε | 1.579 | 1.478 | 0.217 | 1 | 3 | 1 | 2 | 2 | 1 | 24 ± 3 | 34 ± 3 | 63 ± 1 |
| 2007-60-10651-51-2 | O103:H2 | eaeε | 1.284 | 1.804 | 0.344 | 1 | 3 | 1 | 3 | 3 | 2 | 36 ± 2 | 55 ± 7 | 67 ± 3 |
| 2007-60-10709-51-51 | O103:H2 | eaeε | 1.292 | 1.558 | 0.083 | 1 | 3 | 1 | 2 | 2 | 1 | 42 ± 8 | 63 ± 6 | 65 ± 8 |
| 2006-22-1173-53-2 | O103:H25 | eaeγ2/θ | 0.018 | 0.000 | 0.030 | 0 | 1 | 0 | 0 | 0 | 1 | 23 ± 7 | 29 ± 0 | 24 ± 10 |
| 2006-22-1199-51-2 | O103:H25 | eaeγ2/θ | 0.020 | 0.097 | 0.022 | 0 | 1 | 1 | 0 | 1 | 1 | 27 ± 12 | 25 ± 14 | 20 ± 3 |
| 2007-60-10125-53-2 | O26:H11 | eaeβ | 0.388 | 0.301 | 0.061 | 3 | 3 | 2 | 3 | 3 | 2 | 37 ± 6 | 38 ± 7 | 33 ± 5 |
| 2007-60-10710-54-4 | O26:H11 | eaeβ | 0.485 | 0.431 | 0.658 | 3 | 3 | 2 | 3 | 3 | 3 | 39 ± 5 | 39 ± 6 | 38 ± 0 |
| 2007-60-10180-53-2 | O26:H11 | eaeβ | 0.002 | 0.012 | 0.025 | 0 | 0 | 0 | 0 | 0 | 1 | 22 ± 4 | 25 ± 9 | 32 ± 6 |
| 2007-60-10246-55-2 | O26:H11 | eaeβ | 0.000 | 0.019 | 0.017 | 0 | 0 | 0 | 0 | 0 | 1 | 46 ± 3 | 43 ± 1 | 54 ± 3 |
a
There was no variation between parallel assays in the coupon test, i.e., the standard deviation was 0.
b
Negative, isolate did not have the eae gene.