TABLE 2.
Parameter | Result for strain: |
||
---|---|---|---|
EC1118 | K300.1(b) | H2 | |
Main compounds (g/liter) | |||
Consumed sugar | 254.6 ± 0.1 | 254.5 ± 0.0 | 254.7 ± 0.2 |
Ethanol | 118.4 ± 1.2 | 113.6 ± 0.9 | 107.8 ± 0.8 |
Glycerol | 10.8 ± 0.4 | 14.1 ± 0.4 | 17.9 ± 0.8 |
Succinate | 1.3 ± 0.1 | 1.8 ± 0.1 | 1.5 ± 0.1 |
Pyruvate | 0.13 ± 0.01 | 0.16 ± 0.01 | 0.15 ± 0.01 |
Acetate | 0.5 ± 0.1 | 0.1 ± 0.0 | NDb |
Acetaldehyde | 0.016 ± 0.008 | 0.021 ± 0.001 | 0.020 ± 0.006 |
Acetoin | ND | ND | 0.024 ± 0.005 |
2,3-Butanediol | 1.11 ± 0.18 | 1.98 ± 0.38 | 3.93 ± 0.30 |
Yield | |||
EtOH (g/g glucose consumed) | 0.465 ± 0.005 | 0.446 ± 0.003 | 0.423 ± 0.003 |
Glycerol (g/g glucose consumed) | 0.042 ± 0.002 | 0.055 ± 0.000 | 0.070 ± 0.003 |
Glycerol/EtOH (%) | 9.09 ± 0.34 | 12.37 ± 0.05 | 16.57 ± 0.84 |
Ethanol production (% [vol/vol]) | 15.01 ± 0.15 | 14.40 ± 0.11 | 13.67 ± 0.10 |
Glucose (g) required for 1% (vol/vol) ethanol production | 16.99 ± 0.07 | 17.71 ± 0.07 | 18.66 ± 0.08 |
Metabolites were determined by HPLC, GC, or enzymatically. Ethanol was measured by HPLC and by distillation and electronic densitometry. Means and standard deviations of 3 to 4 independent measurements are shown.
ND, not detected (<10 mg/ml).