TABLE 1.
Primers used for amplification and sequencing of Vibrio sp. isolates
| Primer | Sequence (5′→3′) | Gene product | Trimmed amplicon length (bp) | Annealing temp (°C) | Reference |
|---|---|---|---|---|---|
| Vi_gyrBdg2F | GARGTGGTRGATAACTCWATTGATGAAGC | DNA gyrase, β subunit (GyrB) | 570 | 55 | This study |
| VigyrBR | CGGTCATGATGATGATGTTGT | ||||
| VigyrBF | GAAGGTGGTATTCAAGCGTT | ||||
| Vh_gyrB_F | CGTGAGCTTTCTTTCCTAAACTC | ||||
| VipyrHdgF | CCCTAAACCAGCGTATCAACGTATTC | Uridylate kinase (PyrH) | 501 | 55 | This study |
| VipyrHdgR | CGGATWGGCATTTTGTGGTCACGWGC | ||||
| VirecAF | TGCGCTAGGTCAAATTGAAA | Recombinase A (RecA) | 462 | 55 | This study |
| VirecAdgR | GTTTCWGGGTTACCRAACATYACACC | ||||
| Vi_atpAdg_F | ATCGGTGACCGTCARACWGGTAAAAC | ATP synthase, α subunit (AtpA) | 489 | 60 | This study |
| Vi_atpAdg_R | ATACCTGGGTCAACCGCTGG | ||||
| ViatpA-01-F | CTDAATTCHACNGAAATYAGYG | 57 | 26 | ||
| ViatpA-04-R | TTACCARGWYTGGGTTGC |