FIG 5.
Product analysis during biocatalytic application of AlkJ to the oxidation of HLAMe, an industrially relevant intermediate. Oxidation of 0.5 mM HLAMe (circles) to the aldehyde OLAMe (squares), followed by overoxidation to the (di)acid DDAMe (diamonds), was monitored over 60 min, using 0.8 μM AlkJ with Q0 as the electron acceptor. Samples were collected at different time points, and the substrates/products were quantified by gas chromatography analysis after extraction from the aqueous phase.