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. 2014 Apr;80(8):2573–2581. doi: 10.1128/AEM.03995-13

TABLE 1.

Identification of missing proteins in E. coli ATCC 8739 whole-cell proteomes recorded after 30 min of UV-A treatment, TiO2 exposure in the dark, and a photocatalytic test

Functional category Protein identity Gene Location Spot no. Peptides matcheda Coverage (%) pI;b mWc Treatmentd
Lipid transport and metabolism Unnamed protein product fadL Outer membrane 1 3/5 6 5.26; 42,307 UV; C0.1; C0.4
Chain A, X-ray structure of Escherichia coli enoyl reductase with bound Nad and benzodiazaborine fabI Cytoplasm 11 15/18 43 5.58; 27,733 C0.4
Porin Outer membrane protein A ompA Outer membrane 2 11/12 24 5.99; 37,200 P0.1; C0.4
Outer membrane porin protein C ompC Outer membrane 3 18/19 28 4.65; 41,447 P0.1; C0.4
Outer membrane porin protein F ompF Outer membrane 4 18/18 29 4.82; 39,372 P0.1; C0.4
Chain A, outer membrane protein OmpW ompW Outer membrane 5 6/6 10 6.15; 21,675 C0.1; C0.4
Carbohydrate transport and metabolism Maltoporin lamB Outer membrane 6 7/9 10 4.86; 49,968 P0.1; C0.4
Amino acid transport and metabolism Chain A, dipeptide-binding protein complex with glycyl-l-leucine dppA Inner membrane 9 23/29 24 5.74; 57,407 C0.4
Inorganic ion transport and metabolism DNA starvation/stationary-phase protection protein Dps dps Cytoplasm 12 52/54 61 5.70; 18,695 C0.4
DNA starvation/stationary-phase protection protein Dps dps Cytoplasm 13 52/54 61 5.70; 18,695 C0.4
Bacterioferritin, iron storage, and detoxification protein bfr Cytoplasm 14 24/24 69 4.69; 18,495 P0.1; C0.4
Energy production and conversion H+ ATPase F1 alpha subunit atpA Inner membrane 10 22/26 32 5,93; 55,339 C0.1; C0.4
Oxidative stress defense protein Chain A, structure of the hypothetical protein YgiW ygiW Periplasm 7 14/15 45 4.73; 11,905 C0.1; C0.4
Hypothetical protein ECP_2911 yggE Periplasm 8 11/11 28 5.74; 24,981 P0.1; C0.4
Two-component response regulator arcA Cytoplasm 15 12/14 35 5.20; 27,292 P0.1; C0.4
Indigoidine synthase A-like protein IndA-like protein gene Cytoplasm 16 16/16 26 5.29; 32,921 C0.1; C0.4
Putative nucleotide binding protein yajQ Cytoplasm 20 14/15 49 5.94; 18,312 P0.4
Posttranslational modification, protein turnover, chaperones Trigger factor tig Cytoplasm 17 65/70 56 4.73; 48,02 C0.4
DnaK suppressor dksA Cytoplasm 18 3/4 34 4,97; 17,500 C0.1; C0.4
Heat shock protein GrpE grpE Cytoplasm 21 16/18 42 4.68; 21,741 P0.4
Chain A, HslV-HslU Protease HslV gene Cytoplasm 22 5/5 19 5.95; 18,962 P0.4
Translation, ribosomal structure and biogenesis Chain F Ribosomal_S6 Cytoplasm 19 9/10 42 6.58; 11,164 C0.1; C0.4
a

Peptides matched: number of tryptic peptides observed contributing to the percentage of sequence coverage with unique amino acid sequence/total number of unique peptides detected including posttranslational modifications.

b

pI, theoretical isoelectric point.

c

mW, theoretical molecular weight.

d

Nature of the treatment leading to the disappearance of some proteins in 2-DE gels compared to results under standard conditions. UV, 30 min of UV-A irradiation; C0.1 and C0.4, treatment with a cytotoxic effect of TiO2 at 0.1 g/liter and 0.4 g/liter, respectively; P0.1 and P0.4, photocatalytic treatment with TiO2 at 0.1 g/liter and 0.4 g/liter, respectively.