TABLE 1.
Functional category | Protein identity | Gene | Location | Spot no. | Peptides matcheda | Coverage (%) | pI;b mWc | Treatmentd |
---|---|---|---|---|---|---|---|---|
Lipid transport and metabolism | Unnamed protein product | fadL | Outer membrane | 1 | 3/5 | 6 | 5.26; 42,307 | UV; C0.1; C0.4 |
Chain A, X-ray structure of Escherichia coli enoyl reductase with bound Nad and benzodiazaborine | fabI | Cytoplasm | 11 | 15/18 | 43 | 5.58; 27,733 | C0.4 | |
Porin | Outer membrane protein A | ompA | Outer membrane | 2 | 11/12 | 24 | 5.99; 37,200 | P0.1; C0.4 |
Outer membrane porin protein C | ompC | Outer membrane | 3 | 18/19 | 28 | 4.65; 41,447 | P0.1; C0.4 | |
Outer membrane porin protein F | ompF | Outer membrane | 4 | 18/18 | 29 | 4.82; 39,372 | P0.1; C0.4 | |
Chain A, outer membrane protein OmpW | ompW | Outer membrane | 5 | 6/6 | 10 | 6.15; 21,675 | C0.1; C0.4 | |
Carbohydrate transport and metabolism | Maltoporin | lamB | Outer membrane | 6 | 7/9 | 10 | 4.86; 49,968 | P0.1; C0.4 |
Amino acid transport and metabolism | Chain A, dipeptide-binding protein complex with glycyl-l-leucine | dppA | Inner membrane | 9 | 23/29 | 24 | 5.74; 57,407 | C0.4 |
Inorganic ion transport and metabolism | DNA starvation/stationary-phase protection protein Dps | dps | Cytoplasm | 12 | 52/54 | 61 | 5.70; 18,695 | C0.4 |
DNA starvation/stationary-phase protection protein Dps | dps | Cytoplasm | 13 | 52/54 | 61 | 5.70; 18,695 | C0.4 | |
Bacterioferritin, iron storage, and detoxification protein | bfr | Cytoplasm | 14 | 24/24 | 69 | 4.69; 18,495 | P0.1; C0.4 | |
Energy production and conversion | H+ ATPase F1 alpha subunit | atpA | Inner membrane | 10 | 22/26 | 32 | 5,93; 55,339 | C0.1; C0.4 |
Oxidative stress defense protein | Chain A, structure of the hypothetical protein YgiW | ygiW | Periplasm | 7 | 14/15 | 45 | 4.73; 11,905 | C0.1; C0.4 |
Hypothetical protein ECP_2911 | yggE | Periplasm | 8 | 11/11 | 28 | 5.74; 24,981 | P0.1; C0.4 | |
Two-component response regulator | arcA | Cytoplasm | 15 | 12/14 | 35 | 5.20; 27,292 | P0.1; C0.4 | |
Indigoidine synthase A-like protein | IndA-like protein gene | Cytoplasm | 16 | 16/16 | 26 | 5.29; 32,921 | C0.1; C0.4 | |
Putative nucleotide binding protein | yajQ | Cytoplasm | 20 | 14/15 | 49 | 5.94; 18,312 | P0.4 | |
Posttranslational modification, protein turnover, chaperones | Trigger factor | tig | Cytoplasm | 17 | 65/70 | 56 | 4.73; 48,02 | C0.4 |
DnaK suppressor | dksA | Cytoplasm | 18 | 3/4 | 34 | 4,97; 17,500 | C0.1; C0.4 | |
Heat shock protein GrpE | grpE | Cytoplasm | 21 | 16/18 | 42 | 4.68; 21,741 | P0.4 | |
Chain A, HslV-HslU | Protease HslV gene | Cytoplasm | 22 | 5/5 | 19 | 5.95; 18,962 | P0.4 | |
Translation, ribosomal structure and biogenesis | Chain F | Ribosomal_S6 | Cytoplasm | 19 | 9/10 | 42 | 6.58; 11,164 | C0.1; C0.4 |
Peptides matched: number of tryptic peptides observed contributing to the percentage of sequence coverage with unique amino acid sequence/total number of unique peptides detected including posttranslational modifications.
pI, theoretical isoelectric point.
mW, theoretical molecular weight.
Nature of the treatment leading to the disappearance of some proteins in 2-DE gels compared to results under standard conditions. UV, 30 min of UV-A irradiation; C0.1 and C0.4, treatment with a cytotoxic effect of TiO2 at 0.1 g/liter and 0.4 g/liter, respectively; P0.1 and P0.4, photocatalytic treatment with TiO2 at 0.1 g/liter and 0.4 g/liter, respectively.