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EMSAs of different concentrations of AbrC3 protein with ChIP-chip-selected promoters. (A) actII-ORF4p; (B) abrC3p; (C) SCO0736p; (D) absR1p (SCO6992p); (E) SCO2113p; (F) bdtAp (SCO3328p); (G) SCO4709p; (H) SCO6809p. S, specific competition control (adding the corresponding unlabeled specific probe); N, nonspecific competitor DNA (100-fold molar excess of sheared sperm salmon DNA); BSA, specific protein control (4 μM BSA).
