FIG 4.

Identification of a putative AbrC3-binding sequence in different genes. (A) Consensus sequence identified by RSAT consensus alignment of putative promoters* (upstream sequences up to −400 bp plus +100 bp from the translational start point) of genes identified by ChIP-chip and EMSAs. (B) EMSAs of different fragments of the actII-ORF4 promoter* region with 4 μM AbrC3 (the top panel shows the amplified fragment locations in the promoter*). (C) EMSA of different concentrations of the AbrC3 protein with the actII-ORF4 fragment 4 promoter*. S, specific competition control (adding 100-fold molar excess of unlabeled specific probe); N, nonspecific competitor DNA (adding 100-fold excess of sheared sperm salmon DNA); BSA, specific control 2 (adding 4 μM BSA protein).