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Complementation of ΔcasKR restores the ability to grow at low temperature. Five microliters of serial dilutions of the B. cereus wt, ΔcasKR, and ΔcasKR/pHT-casKR strains (grown in liquid medium at 30°C and adjusted to 108 CFU/ml) was spotted on LB agar, and the strains were incubated at 10°C for 20 days.
