TABLE 2.
Primers used in this study
| Oligonucleotide | Sequence (5′–3′)a |
|---|---|
| 5Up-2216-17-Eco | GGCGAATTCGTAGTTGGTGATGAGAATGAAC |
| 3Up-2216-17-Pst | TGGACTGCAGCAATCCTTCAGGTCTTAATTG |
| 5Dn-2216-17-Sal | TGCAGTCGACGAAAACGAGAGAAGAATTTGA |
| 3Dn-2216-17-Bam | GCGGGATCCTTTATCCTGTAAAAACCGATTA |
| Km5out | CGGTATAATCTTACCTATCACC |
| Km3out | TACTCTGATGTTTTATATCTTTTCTAA |
| BC2215-Fw | CCATTTAAATACGTCGGTGT |
| BC2218-Rv | ATATGTTCGGCAATACTTCG |
| 5Up-5412-11 | TCGATGCATGCCATGGACCTGAAAAGGTGGTAAATC |
| 3Up-5412-11 | TTGTCTACAGATTAACTCTTGCTAACCGATTTCTT |
| 5Dn-5412-11 | TCACGGTTTACCCACAGAAACAAATCCCGATGTTA |
| 3Dn-5412-11 | GGGCGATATCGGATCCTTGCGTACGATCAGATAAAT |
| 5spcR | TTAATCTGTAGACAAATTGTGA |
| 3spcR | GTGGGTAAACCGTGAATATC |
| Spc5out | TCACAATTTGTCTACAGATTAA |
| Spc3out | GATATTCACGGTTTACCCAC |
| Cpl2216-17-Fw-Sal | GACGCGTCGACAGGTAGAGCAGACGATTCAA |
| Cpl2216-17-Rv-Bam | GCGGGATCCTTTATCCTGTAAAAACCGATTA |
| qPCR-BC2216-F | AAAAGAGATGCGTCGTTTAG |
| qPCR-BC2216-R | ACACACCAACAGTTTCCTTC |
| qPCR-16S-F | GGTAGTCCACGCCGTAAACG |
| qPCR-16S-R | GACAACCATGCACCACCTG |
a
Restriction enzyme sites are underlined.