FIG 1.

Increase in the culturability of Vibrio spp. in oysters and seawater upon addition of CFS. (A) Log fold change in culturability of Vibrio spp. in oyster tissues after 24 h of treatment with CFS from V. vulnificus C7184 (parent) or JDO1 (luxS mutant) relative to that of control oysters that received artificial seawater (ASW). Wild-type CFS significantly increased the culturability of Vibrio spp. in oysters (one-way ANOVA; P < 0.0001), whereas CFS from the luxS mutant did not. Since HI broth had little effect on culturability, nutrient addition did not affect the culturability of Vibrio spp. Error bars represent SD for 4 oysters. (B) Log fold change in levels of Vibrio spp. in NSW after 24 h (relative to that at 0 h) of treatment with either CFS from V. vulnificus C7184 or the ASW control (unpaired t test; P = 0.0004). Error bars represent SD for 3 NSW samples.