FIG 3.
Schematics of optimized expression vectors (DC_Mag-EGFP and Tet_Mag-EGFP_TetR or Mag-EGFP–EGFP fusion) and chromosomal insertion. Expression vectors contained either the strong optimized PmamDC45 or the inducible Ptet promoter, the optimized oRBS, and the magnetosome anchor mamC, which can be fused via a linker domain to any codon-optimized heterologous gene of interest (GOI). Insertion into the chromosome is via pBAM1-derived insertional plasmids and chromosome expression of fusion proteins.