FIG 1.

EC₅₀ for L. amazonensis intracellular amastigotes. Infected BMDM were treated with tamoxifen (A) or amphotericin B (B) for 48 h at 33°C. The numbers of amastigotes were determined by luciferase quantification. Relative light units for control untreated cells were considered 100%. Values shown are the means and standard deviations of triplicates from one experiment representative of at least three independent experiments. The EC₅₀s and 95% confidence intervals are indicated in the upper right corners.