FIG 2.

Isobolograms representing in vitro interactions between tamoxifen and amphotericin B against L. amazonensis promastigotes (A) and intracellular amastigotes (B). Assays were performed by a fixed-ratio method based on the EC₅₀s, with the combinations being tested at constant ratios of 5:0, 4:1, 3:2, 2:3, 1:4, and 0:5. Results shown are from one experiment performed in triplicate, representative of at least two independent experiments. Plots were compared to a theoretical line that produced a sum of the FICs of 1 at all ratios tested (dashed line), which represents an additive effect of both compounds. The mean sum of the FICs (xΣFIC) for all interactions tested is shown at the upper right corner.