TABLE 1.

Comparison between mutants selected on 0.5 μg/ml versus ≥2 μg/ml moxifloxacin in vitro

Mutation		Data for moxifloxacin at concn (μg/ml) of:
Gene	Amino acid change	0.5	2		3		4
		No. (%)a	No. (%)	Pb	No. (%)	P	No. (%)	P
gyrA	Gly88Cys	0 (0.0)	32 (58.2)	<0.0001	48 (68.6)	<0.0001	61 (88.4)	<0.0001
gyrA	Asp89Asn	1 (1.8)	0 (0.0)	1	0 (0.0)	0.4444	0 (0.0)	0.4480
gyrA	Ala90Lys	1 (1.8)	0 (0.0)	1	0 (0.0)	0.4444	0 (0.0)	0.4480
gyrA	Ala90Val	1 (1.8)	0 (0.0)	1	0 (0.0)	0.4444	0 (0.0)	0.4480
gyrA	Asp94Asn	5 (8.9)	10 (18.2)	0.1757	14 (20.0)	0.1315	7 (10.1)	1
gyrA	Asp94Gly	5 (8.9)	3 (5.5)	0.7163	4 (5.7)	0.5093	0 (0.0)	0.0163
gyrA	Asp94His	3 (5.4)	1 (1.8)	0.6182	0 (0.0)	0.0852	0 (0.0)	0.0872
gyrA	Asp94Lys	0 (0.0)	0 (0.0)	NAc	0 (0.0)	NA	1 (1.4)	1
gyrA	Asp94Tyr	14 (25)	9 (16.4)	0.3497	4 (5.7)	0.0038	0 (0.0)	<0.0001
gyrB	Asn499Asp	2 (3.6)	0 (0.0)	0.4955	0 (0.0)	0.1956	0 (0.0)	0.1987
gyrBd	Asn499Lys	17 (30.4)	0 (0.0)	<0.0001	0 (0.0)	<0.0001	0 (0.0)	<0.0001
gyrB	Thr500Asn	6 (10.7)	0 (0.0)	0.0271	0 (0.0)	0.0066	0 (0.0)	0.0069
gyrB	Glu501Asp	1 (1.8)	0 (0.0)	1	0 (0.0)	0.4444	0 (0.0)	0.4480

^aPercentage of all mutants selected at the indicated moxifloxacin concentration.

^bSignificance of proportion compared to that at 0.5 μg/ml by Fisher's exact test; values in bold type are significant (P < 0.05).

^cNA, not applicable.

^dNumbering for gyrB according to Maruri et al. (22).