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Relative activities of PhaCAc and PhaCRe for the R-3HB-CoA substrate at different concentrations of PhaPAc and PhaP1Re. Relative activities were determined using the change in absorbance at 236 nm at maximum velocity. Reaction mixtures containing PhaC (350 nM PhaCAc without a His tag or 200 nM PhaCRe), 100 μM R-3HB-CoA, and 0 to 5 μM PhaP (PhaPAc or PhaR1Re) were used. Results are expressed as the means ± standard errors (n = 3), except for results with 5 μM PhaP (n = 2).
