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Western blots of PhaCs in 6-h-cultured PhaCAc-expressing strains (a) and PhaCRe-expressing strains (b) with and without PhaP expression. The l-arabinose concentration was 1.0% (wt/vol). Lanes 1, 3, 5, 7, 9, and 11 are PhaPAc-free strains. Lanes 2, 4, 6, 8, 10, and 12 are PhaPAc-expressing strains. The soluble cell extract, containing PHA granules, and the insoluble precipitate were separated by low-speed centrifugation (1,500 × g, 5 min, 4°C). The amount of protein in each lane was 8 μg.
