TABLE 1.
PhaC activity assay toward R-3HB-CoA in the presence of PhaPa
| Assay conditions | Sp act (mean U/mg PhaC ± SE) (fold activation by PhaP) of: |
|||
|---|---|---|---|---|
| PhaCAc |
PhaCRe | PhaCDa | ||
| Without His tag | With His tag | |||
| PhaP free | 0.76 ± 0.08 (1.0) | 0.72 ± 0.03 (1.0) | 3.51 ± 0.21 (1.0) | 1.40 ± 0.14 (1.0) |
| +PhaPAc | 2.31 ± 0.07 (3.0) | 2.24 ± 0.11 (3.1) | 0.36 ± 0.07 (0.1) | 0.15 ± 0.03 (0.1) |
| +PhaP1Re | 1.82 ± 0.10 (2.4) | 1.76 ± 0.03 (2.4) | 0.39 ± 0.02 (0.1) | 0.64 ± 0.06 (0.5) |
a
Assay conditions: PhaCs, 350 nM (PhaCAc with/without His tag) or 200 nM (PhaCRe and PhaCDa with a His tag); R-3HB-CoA, 100 μM; PhaP, 0 or 1 μM. n = 3 replicates.