TABLE 3.
P(3HB) synthesis in recombinant E. coli TOP10 cells cultured in the presence of l-arabinosea
| Expressed protein(s) | Dry cell wt (g/liter) | P(3HB) content (wt%) | Mn (×105) | Mw (×105) | Mw/Mn |
|---|---|---|---|---|---|
| PhaCAc | 1.2 ± 0.1 | 17 ± 2 | 3.5 ± 0.6 | 12.0 ± 0.9 | 3.4 |
| PhaCAc + PhaPAc | 2.1 ± 0.4 | 39 ± 4 | 2.4 ± 0.6 | 7.4 ± 0.5 | 3.1 |
| PhaCRe | 1.9 ± 0.1 | 35 ± 2 | 24.9 ± 3.5 | 39.9 ± 6.3 | 1.6 |
| PhaCRe + PhaPAc | 2.1 ± 0.1 | 43 ± 1 | 9.3 ± 1.1 | 24.4 ± 1.4 | 2.6 |
a
Strains were the same as in the experiments whose results are shown in Fig. 6. The l-arabinose concentration was 1.0% (wt/vol). Results are expressed as the means ± standard errors (n = 3).