FIG 4.

Expression of aglycosylated LST from P. pastoris. (A) Expression of aglycosylated LST variants. Lanes: M, protein marker (top to bottom, 250, 150, 100, 75, 50, 37, 25, and 20 kDa); 1, LST; 2, LST(N125Q); 3, LST(N125D); 4, LST(N125S); 5, LST(S126P); 6, LST(T127A). All variants exhibited similar expression levels. (B) Activity assays of aglycosylated LST variants. MIC assays with S. aureus SA113 were performed on 2-fold serial dilutions of culture supernatants. The OD₆₅₀ readings for each enzyme dilution series are shown, and a dashed line indicates the reading corresponding to the MIC₅₀. Error bars represent standard deviations.