TABLE 1.

Primers used in this study

Primer	Sequence (5′ to 3′)
WT-F	ATCGCTCGAGAAAAGAGCTGCAACACAT
WT-R	CGATGAATTCTTACTTTATAGTTCCCCA
Chi1-F	CAAAGAATGGTTAATTCATTTTCCCAATCCACCGCTCAAGAC
Chi1-R	GTCTTGAGCGGTGGATTGGGAAAATGAATTAACCATTCTTTG
Chi2-F	CAAAGAATGGTCAACTCCTTCTCAAATTCAACTGCCCAAGAT
Chi2-R	ATCTTGGGCAGTTGAATTTGAGAAGGAGTTGACCATTCTTTG
Chi3-F	GAAGCTGGTTGGAGTAATTACGGAGGTGGTAACCAAATCGGTTTGATC
Chi3-R	GATCAAACCGATTTGGTTACCACCTCCGTAATTACTCCAACCAGCTTC
Chi4-F	GAGGCTGGTTGGTCCAACTACGGTGGAGGTAATCAAATAGGTCTTATT
Chi4-R	AATAAGACCTATTTGATTACCTCCACCGTAGTTGGACCAACCAGCCTC
Chi5-F	AAATATAATGTTAAAGTAGGAGATTACGTCAAGGCTGGTCAAATCATC
Chi5-R	GATGATTTGACCAGCCTTGACGTAATCTCCTACTTTAACATTATATTT
Chi6-F	AAGTACAACGTCAAGGTCGGTGACTATGTCAAAGCTGGTCAAATAATC
Chi6-R	GATTATTTGACCAGCTTTGACATAGTCACCGACCTTGACGTTGTACTT
Chi7-F	GGTCTTATTGAAAATGATGGAGTGCACAGACAATGGTACATGCACTTG
Chi7-R	CAAGTGCATGTACCATTGTCTGTGCACTCCATCATTTTCAATAAGACC
Chi8-F	GGTTTGATCGAGAACGACGGTGTCCATAGACAATGGTATATGCATCTA
Chi8-R	TAGATGCATATACCATTGTCTATGGACACCGTCGTTCTCGATCAAACC
Syn-F	ATCGCTCGAGAAAAGAGCTGCTACCCAC
Syn-R	CGATGAATTCTTACTTGATGGTACCCCA
N125Q-F	AACTCCTTCTCCCAATCCACCGCTCAA
N125Q-R	TTGAGCGGTGGATTGGGAGAAGGAGTT
N125D-F	AACTCCTTCTCCGACTCCACCGCTCAA
N125D-R	TTGAGCGGTGGAGTCGGAGAAGGAGTT
N125S-F	AACTCCTTCTCCTCCTCCACCGCTCAA
N125S-R	TTGAGCGGTGGAGGAGGAGAAGGAGTT
S126P-F	AACTCCTTCTCCAACCCAACCGCTCAA
S126P-R	TTGAGCGGTTGGGTTGGAGAAGGAGTT
T127A-F	TTCTCCAACTCCGCTGCTCAAGACCCA
T127A-R	TGGGTCTTGAGCAGCGGAGTTGGAGAA
Alfa-F	ATGAGATTTCCTTCAATTTTTACTG
5Syn-R	CACCGTCGTTCTCGATCAAACCGA
FullSyn-R	CTTGATGGTACCCCACAAGACACC
5WT-R	CTCCATCATTTTCAATAAGACCTA
FullWT-R	CTTTATAGTTCCCCAAAGAACACC
AOX1-F	ATTGCCGGAAGATTGGCAAACTTG
AOX1-R	AAAACGATTTGCTTTCTAGCACGG
N125NNK-F	AACTCCTTCTCCNNKTCCACCGCTCAA
N125NNK-R	TTGAGCGGTGGAMNNGGAGAAGGAGTT