TABLE 3.
Mass spectrometry and top-down proteomic identification
| Strain | Stx2 subtype(s) | A2 fragment |
B subunit |
||||
|---|---|---|---|---|---|---|---|
| DNA sequencing (Da) | MS (m/z) | MS/MS top-down identification, P (%), subtypes | DNA sequencing (Da) | MS (m/z) | MS/MS top-down identification, P (%), subtype(s) | ||
| RM14735 | a | 5,285 | 5,291 | 7.9E−17 (57.97), a, c | 7,818 | 7,819 | 1.4E−16 (61.36), a |
| RM12509 | a | 5,285 | 5,284 | 4.0E−21 (53.23), a, c | 7,818 | 7,817 | 2.3E−14 (52.94), a |
| RM10638 | a | 5,285 | 5,286 | 5.0E−10 (31.25), a, c | 7,818 | 7,816 | 4.6E−9 (43.48), a |
| RM13368 | a | 5,226 | 5,226 | 3.0E−16 (48.57), a, d, e | 7,818 | 7,817 | 1.4E−12 (38.78), a |
| RM7787 | c | 5,285 | 5,290 | 1.5E−10 (43.94), a, c | 7,773 | 7,774 | 1.7E−19 (42.70), c, d |
| RM8091 | c | 5,285 | 5,284 | 3.0E−11 (42.62), a, c | 7,773 | 7,773 | 5.2E−15 (45.33), c, d |
| RM9482 | c | 5,285 | 5,286 | 1.3E−14 (50.00), a, c | 7,773 | 7,775 | 1.7E−18 (51.39), c, d |
| RM9483 | c | 5,285 | 5,284 | 3.2E−5 (35.14), a, c | 7,773 | 7,773 | 1.5E−14 (46.38), c, d |
| RM10058 | a and c | 5,285 | 5,288 | 7.6E−9 (38.64), a, c | 7,773 | 7,775 | 3.7E−11 (33.64), c, d |
| RM10936 | c | 5,285 | 5,284 | 4.8E−11 (37.10), a, c | 7,773 | 7,773 | 1.9E−13 (46.77), c, d |
| RM10648 | c | 5,271 | 5,269 | 2.8E−7 (35.09), a, c | 7,773 | 7,772 | 6.6E−16 (47.89), c, d |
| RM7975 | c | 5,271 | 5,271 | 1.3E−8 (33.93), a, c | 7,773 | 7,774 | 1.0E−11 (38.10), c, d |
| RM8017 | c | 5,271 | 5,271 | 4.5E−7 (36.36), a, c | 7,773 | 7,774 | 2.4E−11 (57.58), c, d |
| RM6976 | c | 5,271 | 5,275 (weak), a, c | 7,773 | 7,778 | 1.7E−10 (34.02), c, d | |
| RM6977 | c | 5,271 | 5,275 (weak), a, c | 7,773 | 7,777 | 9.1E−9 (50.00), c, d | |
| RM6978 | c | 5,271 | 5,273 (weak) | 2.0E−4 (23.08), a, c | 7,773 | 7,779 | 1.6E−6 (20.45), c, d |
| RM12508 | c and/or d | 5,226 | 5,224 | 4.8E−11 (34.72), a, d, e | 7,773 | 7,772 | 1.3E−11 (23.88), c, d |
| RM7006 | d | 5,226 | NDa | 7,773 | 7,772 (weak) | ||
| RM13503 | d | 5,226 | 5,224 | 5.4E−13 (32.39), a, d, e | 7,773 | 7,773 | 8.0E−22 (53.85), c, d |
| RM8013 | d | 5,226 | 5,227 | 6.7E−6 (20.90), a, d, e | 7,715 | 7,714 | 3.9E−12 (37.78), d |
| RM9508 | f | 5,238 | ND | 7,555 | 7,552 | 2.2E−7 (13.21), f | |
| RM9511 | f | 5,238 | ND | 7,555 | 7,556 | 2.1E−4 (10.94), f | |
| RM9973 | f | 5,238 | ND | 7,555 | 7,556 | 1.6E−7 (14.63), f | |
| RM9974 | f | 5,238 | ND | 7,555 | 7,560 | 2.0E−6 (16.67), f | |
| RM9981 | f | 5,176 | ND | 7,582 | 7,581 (weak) | ||
| RM10468 | g | 5,348 | 5,348 (weak), g | 7,799 | 7,799 | 2.5E−6 (23.81), g | |
a
ND, not detected.