FIG 2.

Biphasic kinetics of l-arabitol transport into A. monospora. The initial transport rates (V) were measured between 0.05 and 50 mM l-arabitol. The Eadie-Hofstee plot shows experimental points (●) fitted by curves calculated for the sum of either two Michaelis-Menten systems, a high-affinity system with a K_m of 0.16 mM and a V_max of 3.9 μmol · min⁻¹ · g (dry weight) yeast⁻¹ plus a low-affinity system with a K_m of 75 mM and a V_max of 49 μmol · min⁻¹ · g (dry weight) yeast⁻¹ (dashed line) or the same high-affinity system plus a first-order system with a k of 0.55 μmol · min⁻¹ · g (dry weight) yeast⁻¹ · mM l-arabitol⁻¹ (dotted line).