FIG 4.

Effect of C-terminal tags on the transport activities of Lat1 and Lat2. Initial transport rates were assayed at 10 mM or 0.2 mM pentitol or 5 mM l-arabinose (labeled with repurified l-[1-³H]arabinose). Error bars show the SDs of 2 to 7 independent replicates. Control strains include both untransformed hosts and hosts carrying empty plasmids. Transformants carried plasmids containing the unmodified transporter genes (pLAT1 and pLAT2) or transporter genes fused in frame to sequences encoding red fluorescent protein, green fluorescent protein, or adenylate kinase (pLAT1-mCherry, pLAT2-mCherry, pLAT2-GFP, or pLAT2-AK). A. monospora was grown on 40 g l-arabinose · liter⁻¹, and the other yeasts were grown on 20 g glucose · liter⁻¹.