TABLE 1.
Genetically engineered S. cerevisiae strains used in this studya
| Strain | Relevant gene(s) | Plasmid(s) | Host | Reference |
|---|---|---|---|---|
| H2984 | 5 integrated genes for l-arabinose pathway | None | CEN.PK2-1D | Verho et al. (11) |
| H3169 | pLAT1 | pFL60 | H2984 | Verho et al. (11) |
| H3187 | pLAT2 | pFL60 | H2984 | Verho et al. (11) |
| H4387 | pLAT1 and pLAT2 | B2158 and B2159 | CEN.PK2-1D | This work |
| H4388 | pLAT1-mCherry | B2158 | CEN.PK2-1D | This work |
| H4389 | None (control) | B2158 | CEN.PK2-1D | This work |
| H4390 | pLAT2-GFP | B2159 | CEN.PK2-1D | This work |
| H4391 | None (control) | B2159 | CEN.PK2-1D | This work |
| H4392 | pLAT1-mCherry and pLAT2-GFP | B2158 and B2159 | CEN.PK2-1D | This work |
| H4393 | None (control) | B2158 and B2159 | CEN.PK2-1D | This work |
| H4394 | LAT2-GFP | M4297 | CEN.PK2-1D | This work |
| H4395 | LAT2-GFP | M4297 | H2984 | This work |
| H4399 | LAT2 partial deletant | M4297 | CEN.PK2-1D | This work |
| H4400 | LAT2-1/2GFP | M4297 | CEN.PK2-1D | This work |
| H4402 | pLAT2-mCherry | B2158 | CEN.PK2-1D | This work |
| H4403 | pLAT2-AK | B2158 | CEN.PK2-1D | This work |
a
Multicopy plasmids (pFL60, B2158, and B2159) also carried the indicated genes. Integration vector M4297 targeted the indicated genes at the S. cerevisiae HO locus.