TABLE 3.
Transport of l-arabinose, l-arabitol, and ribitol by Ambrosiozyma monosporaa
| Substrate | Concn (mM) | Transport rateb for yeast grown on: |
|
|---|---|---|---|
| Glucose | l-Arabinose | ||
| l-[1-14C]arabinose (ARC lot 110523) | 20 | 0.24 ± 0.01 | 22.7 ± 8.4 |
| Repurified l-[1-3H]arabinose | 20 | NA | 19.1 ± 4.0 |
| Repurified l-[1-3H]arabinose | 5 | NA | 17.9 ± 6.1 |
| l-[14C]arabitol | 10 | 0.0 ± 0.3 | 12.9 ± 3.2 |
| 0.2 | NA | 3.1 ± 1.1 | |
| [14C]ribitol | 10 | 0.42 ± 0.08 | 14.1 ± 4.9 |
| 0.2 | NA | 4.3 ± 0.4 | |
a
Yeast was grown on YNB containing glucose (20 g · liter−1) or l-arabinose (40 g · liter−1).
b
Transport rates (in micromoles · minute−1 · gram [dry weight] yeast−1) are means ± standard deviations (SDs) (n = 2 for glucose-grown yeast; n = 2 to 6 for l-arabinose-grown yeast). NA, not analyzed.