TABLE 4.
Transport of ribitol and l-arabitol by control strains and transformants carrying LAT1 or LAT2
| Substrate | Concn (mM) | Transport ratea |
|||
|---|---|---|---|---|---|
| Host strains | Empty plasmidb | H3169 (pLAT1) | H3187 (pLAT2) | ||
| l-Arabitol | 10 | 0.04 ± 0.02 | 0.05 ± 0.06 | 0.38 ± 0.13 | 0.11 ± 0.07 |
| 0.2 | NA | 0.01 | 0.01 ± 0.00 | 0.02 ± 0.01 | |
| Ribitol | 10 | 0.04 ± 0.06 | 0.06 ± 0.09 | 0.33 ± 0.08 | 0.18 ± 0.02 |
| 0.2 | NA | −0.02 | 0.00 ± 0.00 | 0.02 ± 0.00 | |
a
Initial rates of transport (μmol · min−1 · g [dry weight] yeast−1) were measured with l-[14C]arabitol and [14C]ribitol at the indicated concentrations. Host strains include both strain CEN.PK2-1D and strain H2984, which had similar activities. Data are means ± SDs (n = 2 to 7) or single experiments where no SDs are shown. NA, not analyzed.
b
Strain CEN.PK2-1D carrying the empty plasmids, B2158 and B2159.