FIG 3.

malE1 expression level (A), Western blot analysis of ME1 (B), specific activity (C), NADPH level (D), and cell fatty acid composition (E) in the control strain MAU1 and overexpressing strain MA-malE1-1. Samples were taken at the indicated times from cultures of both strains growing in Kendrick medium at an initial pH of 6.0 in a stirred 2-liter fermentor with direct aeration for up to 144 h at 28°C. In panels A, C, and D, the open bars represent the control strain MAU1 and the black bars represent the malE1 overexpression strain MA-malE1-1. In panel E, the dark gray bars represent AA, the light gray bars represent other ω6 PUFAs (linoleic acid, γ-linolenic acid, and dihomo-γ-linolenic acid), and the open bars represent other fatty acids. Three independent experiments were performed, and error bars represent standard deviations. n.s., not significant. *, P < 0.05 compared with the control.