TABLE 3.
OTU of bacteria that were highly enriched on soil-derived J2 of M. hapla compared to the bacterial community in soil, based on 16S rRNA gene amplicon pyrosequencing
| Most similar cultured species or environmental sequence of the OTU specific for J2 (GenBank accession no., % identity)a | No. of sequences |
||
|---|---|---|---|
| J2 from Kw | J2 from Gb | J2 from Go | |
| Micrococcus yunnanensis (KC469953, 100) | 9 | 21 | 612 |
| Rothia amarae (T) (AY043359, 100) | 835 | 0 | 0 |
| Geobacillus stearothermophilus (T) (AB021196, 99.2) | 394 | 74 | 0 |
| Streptococcus salivarius (T) (AY188352, 100) | 0 | 651 | 0 |
| Anaerococcus octavius (T) (Y07841, 99.2) | 91 | 4 | 177 |
| Peptoniphilus gorbachii (T) (DQ911241, 100) | 118 | 0 | 28 |
| Clostridium disporicum (T) (Y18176, 99.6) | 202 | 3 | 0 |
| Mycoplasma wenyonii (CP003703, 99.7) | 110 | 1 | 3 |
| Uncultured Gemmatimonas in rhizosphere (EU159980, 98.9) | 101 | 1 | 0 |
| Uncultured deltaproteobacterium (HE613616, 100) | 96 | 3 | 0 |
| Ochrobactrum sp./Brucella sp. (AJ242584/AY594216, 99.8) | 147 | 17 | 0 |
| Hirschia maritima (T) (FM202386, 96.0) | 128 | 0 | 0 |
| Haematobacter missouriensis (T) (DQ342315, 100) | 222 | 0 | 0 |
| Paracoccus yeei (T) (AY014173, 100) | 161 | 0 | 0 |
| Uncultured Rhodospirillaceae (GQ263062, 100) | 261 | 5 | 0 |
| Malikia spinosa (AB077038, 98.5) | 962 | 0 | 48 |
| Janthinobacterium lividum (T) (Y08846, 99.8) | 480 | 13 | 0 |
| Neisseria mucosa (HG005351, 99.8) | 104 | 0 | 0 |
| Vogesella indigofera (AB021385, 99.2) | 0 | 421 | 0 |
| Shigella flexneri/S. fergusonii (T) (X96963/AF530475, 100) | 518 | 0 | 109 |
| Acinetobacter schindleri (T) (AJ278311, 99.6) | 0 | 76 | 305 |
| Acinetobacter johnsonii (X81663, 100) | 0 | 229 | 67 |
| Enhydrobacter aerosaccus (T) (AJ550856, 100) | 172 | 3 | 67 |
| Pseudomonas kilonensis (T) (NR_028929, 100) | 281 | 9 | 0 |
| Total sequences | 7,647 | 8,664 | 6,164 |
a
That is, OTU that comprised >1% of sequences from all J2 or only from Kw-J2 samples and that were not detected in soil or had at least a 100-fold higher relative abundance on J2 compared to soil. Sequence data from soil were obtained from NCBI sequence read archive study accession number SRP029944. Details for the BLASTN results and taxonomy are given in the supplemental material. (T), type strain.