FIG 2.

(A) Coomassie brilliant blue-stained SDS-PAGE gel of surface proteins extracted from the tagE deletion mutants as well as from the wild type and acm2 deletion mutant to detect Acm2 (indicated by the arrow). (B) sWGA, Dolichos biflorus lectin, and Lens culinaris lectin blots of whole-cell extracts derived from the tagE deletion mutants, Lactobacillus plantarum WCFS1 (wild type), and the acm2 deletion mutant to assess glycan moieties. On the left side of the blot the protein sizes are indicated based on the Precision Plus protein dual color standard (Bio-Rad) molecular marker (data not shown). M, CandyCane glycoprotein molecular size marker. The arrow indicates Acm2.