TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristic(s)a | Reference or sourceb |
|---|---|---|
| Strains | ||
| L. plantarum | ||
| WCFS1 | Single-colony isolate of L. plantarum NCIMB8826 | 46 |
| ATCC 14917 | Isolate from pickled cabbage | ATCC |
| ATCC 8014 | Isolate from maize ensilage | ATCC |
| CIP104440 | Isolate from human stool | CIP |
| CIP104450 | Isolate from human stool | CIP |
| NC8 | Isolate from grass silage | 77 |
| NCIMB12120 | Isolate from Ogi, Nigeria | NCIMB |
| LP80 | Isolate from silage | 78 |
| LP85-2 | Origin from silage, France | 57 |
| Δacm2 mutant | NZ3557Cm; Cmr; derivative of WCFS1 containing a lox66-P32-cat-lox71-tagH9 replacement of acm2 (acm2::lox66-P32-cat-lox71-tagH9) | 41 |
| ΔtagE1 mutant | NZ3540Cm; Cmr; derivative of WCFS1 containing a lox66-P32-cat-lox71-tag8.5 replacement of tagE1 (tagE1:: lox66-P32-cat-lox71-tag8.5) | This study |
| ΔtagE2E3 mutant | NZ3541Cm; Cmr; derivative of WCFS1 containing a lox66-P32-cat-lox71-tagF10 replacement of tagE2E3 (tagE2E3::lox66-P32-cat-lox71-tagF10) | This study |
| ΔtagE4 mutant | NZ3542Cm; Cmr; derivative of WCFS1 containing a lox66-P32-cat-lox71-tagG1 replacement of tagE4 (tagE4:: lox66-P32-cat-lox71-tagG1) | This study |
| ΔtagE5E6 mutant | NZ3543Cm; Cmr; derivative of WCFS1 containing a lox66-P32-cat-lox71-tagG7 replacement of tagE5E6 (tagE5E6::lox66-P32-cat-lox71-tagG7) | This study |
| tagE5E6 complementation mutant | NZ8204CmEm; Cmr Emr; derivative of NZ3543Cm containing chromosomally integrated pNZ8204 at tRNASer site | This study |
| tagE6 complementation mutant | NZ8205CmEm; Cmr Emr; derivative of NZ3543Cm containing chromosomally integrated pNZ8205 at tRNASer site | This study |
| tagE5 complementation mutant | NZ8206CmEm; Cmr Emr; derivative of NZ3543Cm containing chromosomally integrated pNZ8206 at tRNASer site | This study |
| E. coli | ||
| TOP 10 | Cloning host; F− mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| Plasmids | ||
| pNZ5319 | Cmr Emr; mutagenesis vector for gene replacements in L. plantarum | 50 |
| pNZ3540 | Cmr Emr; pNZ5319 derivative containing homologous regions up- and downstream of tagE1 | This study |
| pNZ3541 | Cmr Emr; pNZ5319 derivative containing homologous regions up- and downstream of tagE2-E3 | This study |
| pNZ3542 | Cmr Emr; pNZ5319 derivative containing homologous regions up- and downstream of tagE4 | This study |
| pNZ3543 | Cmr Emr; pNZ5319 derivative containing homologous regions up- and downstream of tagE5E6 | This study |
| pMEC10 | Emr; integration plasmid | 79 |
| pNZ8204 | Emr; pMEC10 derivative harboring tagE5E6 | This study |
| pNZ8205 | Emr; pMEC10 derivative harboring tagE6 | This study |
| pNZ8206 | Emr; pMEC10 derivative harboring tagE5 | This study |
a
Cmr, chloramphenicol resistant; Emr, erythromycin resistant; Strr, streptomycin resistant.
b
ATCC, American Type Culture Collection, Manassas, VA; CIP, Collection de l'Institut Pasteur, Paris, France; NCIMB, National Collections of Industrial, Food and Marine Bacteria, Aberdeen, Scotland.