TABLE 2.
Primers used in this study
| Primer | Sequencea | Reference or source |
|---|---|---|
| is128 tag-lox66-F3 | 5′-AAATCTACCGTTCGTATAATGTATG-3′ | 80 |
| is129 tag-lox71-R3 | 5′-CTCATGCCCGGGCTGTAACCG-3′ | 80 |
| 87 | 5′-GCCGACTGTACTTTCGGATCC-3′ | 50 |
| CreF | 5′-CGATACCGTTTACGAAATTGG-3′ | 50 |
| CreR | 5′-CTTGCTCATAAGTAACGGTAC-3′ | 50 |
| EryintF | 5′-TCAAATACAGCTTTTAGAACTGG-3′ | 50 |
| EryintR | 5′-ATCACAAACAGAATGATGTACC-3′ | 50 |
| tagE1-Up-F | 5′-GCCGCAACAACCATACTGGG-3′ | This study |
| tagE1-Up-R | 5′-GCATACATTATACGAACGGTAGATTTAAAATAATACATCACCTAGCCG-3′ | This study |
| tagE1-Down-F | 5′-CGGTTACAGCCCGGGCATGAGATAGCAGCACTTTAAGAACTGG-3′ | This study |
| tagE1-Down-R | 5′-GCGATTACATCGCCTTGGCG-3′ | This study |
| tagE1-out-F | 5′-GCTAGTCATGTCACGGATGC-3′ | This study |
| tagE1-out-R | 5′-TCACTCACAATAAATTCCCCC-3′ | This study |
| tagE2E3-Up-F | 5′-GCAATTACAATGTTGTGCGGC-3′ | This study |
| tagE2E3-Up-R | 5′-GCATACATTATACGAACGGTAGATTTGAAGTAAAACATACAGTCACCC-3′ | This study |
| tagE2E3-Down-F | 5′-CGGTTACAGCCCGGGCATGAGACGGCTTAAGTAGATTTGACGG-3′ | This study |
| tagE2E3-Down-R | 5′-AAGTGCGCGTTTTAGTACGC-3′ | This study |
| tagE2E3-out-F | 5′-TACGGTTATTTTCCGGCTCG-3′ | This study |
| tagE2E3-out-R | 5′-ATCGGTGGCCTTTTACTTGG-3′ | This study |
| tagE4-Up-F | 5′-CGTATCGATTGTTGACAGCG-3′ | This study |
| tagE4-Up-R | 5′-GCATACATTATACGAACGGTAGATTTATCGGCTAAACAACCACATGC-3′ | This study |
| tagE4-Down-F | 5′-CGGTTACAGCCCGGGCATGAGGAAATACATTTGCTACGCCCC-3′ | This study |
| tagE4-Down-R | 5′-CGAAGTGACGACTGCAAACG-3′ | This study |
| tagE4-out-F | 5′-CTTTCGTAGCCAAAATCGACG-3′ | This study |
| tagE4-out-R | 5′-CAAGAACAAGTCACAGCCGC-3′ | This study |
| tagE5E6-Up-F | 5′-ATTGGAAACGTTCTGTGCGG-3′ | This study |
| tagE5E6-Up-R | 5′-GCATACATTATACGAACGGTAGATTTGTTGTTCAGTGAATATCAAAAATGG-3′ | This study |
| tagE5E6-Down-F | 5′-CGGTTACAGCCCGGGCATGAGATAATACATTATTACTCGCTCCC-3′ | This study |
| tagE5E6-Down-R | 5′-AGTTGTTGATGAACTGCTGC-3′ | This study |
| tagE5E6-out-F | 5′-AAATAATAGTTAGGGGTGAACAC-3′ | This study |
| tagE5E6-out-R | 5′-CTTCAGCACTACTTGATGTGC-3′ | This study |
| tRNA | 5′-GCGAACCGGCTAATACCGGC-3′ | 81 |
| IC013 | 5′-AGCTAACAGACCGGTAGCTGCCAATGAAG-3′ | This study |
| IC014 | 5′-AACCAGAGCTCCTGGCTGCTACGTGAACCTAATTCC-3′ | This study |
| IC015 | 5′-TTTCCGAGCTCGCGTTACTAGTTTAGCCGGTGCCTG-3′ | This study |
| IC016 | 5′-TATTGGTTCACAAAAAAATTCATTATTACTCGCTCCCTTACACGA-3′ | This study |
| IC017 | 5′-CGTGTAAGGGAGCGAGTAATAATGAATTTTTTTGTGAACCAATATT-3′ | This study |
| IC021 | 5′-ACGCCACATGCAGTCGATCC-3′ | This study |
| IS169 | 5′-TTATCATATCCCGAGGACCG-3′ | 82 |
| IS247 | 5′-AGATTGTACTGAGAGTGCACC-3′ | This study |
| IS260 | 5′-GTTGAAAGAACCTGTACTCTCC-3′ | This study |
a
Underlined nucleotides indicate parts of the primers that are complementary to the is128-lox66-F3 and is129-lox71-R3 primers.