FIG 4.

SDS-PAGE of proteins secreted into the culture medium (Sup) by SJW2219 and UA002 cells (Cell). Cells were grown in TY medium at a temperature (Temp) of either 30 or 37°C. Secreted proteins were recovered and examined as described in the legend to Fig. 3. Supernatants from SJW2219 are in lanes 1 and 3 (grown at 30 and 37°C, respectively), and those from UA002 are in lanes 2 and 4 (grown at 30 and 37°C, respectively). Proteins from whole SJW2219 cells are in lanes 5 and 7, and those from whole UA002 cells are in lanes 6 and 8 (grown at 30 and 37°C, respectively).