TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype | Source or reference |
|---|---|---|
| Strains | ||
| SJW1103 | Wild type | S. Yamaguchi |
| SJW2219 | flgE2219 (T149N) | 9 |
| UA001 | flgE2219 (T149N) fliK6125 [FliK95′-YscP (217-381)-′96FliK] | This study |
| UA002 | flgE2219 (T149N)/pTrc99AFF4-fliC | This study |
| SJW2536 | ΔfliC | S. Yamaguchi |
| SJW108 | ΔfliK | S. Yamaguchi |
| TH16783 | ΔaraBAD7606::fliK+ ΔfliK6140 | M. Erhardt |
| TH18945 | flgM5794::FRT flgE2219 (T149N) fljBenx fljBenxvh2 | K. Hughes |
| Plasmids | ||
| pTH8256a | FliK95::YscP 217-381 | 13 |
| pAH25 | pTrc99AFF4-fliC | 16 |
a
Plasmid pTH8256 was used to construct UA001 by recombination.