TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| E. coli S17-1 | recA hsdR thi pro ara RP-4 2-Tc::Mu Km::Tn7 (Tpr Smr) | 42 |
| E. coli SM10λpir | thi thr leu tonA lacY supE recA::RP-4 2-Tc::Mu Km λpirRK6 | 43 |
| V. alginolyticus VIO5 | Rifr Pof+ Laf− Pom+ | 28 |
| V. alginolyticus NMB198 | VIO5 ΔfliG (Rifr Pof− Pom−) | 18 |
| V. alginolyticus NMB301 | VIO5 ΔpomAB ΔfliG (Rifr Pof− Pom−) | This study |
| Plasmids | ||
| pKY704 | Suicide vector, Cmr | 44 |
| pKY704-ΔpomAB | pKY704-pomA1–68-pomB126–301, which was constructed by the DraI and HpaI deletion | 29 |
| pKY704-ΔpomAB-sacB | pKY704-ΔpomAB, 2.0-kb XbaI-XbaI fragment (sacB+) from pHFS401 | Hiroyuki Terashima |
| pSU41 | Kmr, Plac | 45 |
| pYA303 | pSU41-pomAB | 46 |
| pBAD33 | Cmr, PBAD | 47 |
| pHFGBA2 | pBAD33-his6-egfp-pomB, pomA | 26 |
| pMMB206 | Cmr, Ptac PlacUV5 | 48 |
| pNT1 | pMMB206-fliG | This study |
| pTY60 | Kmr, PBAD | 24 |
| pNO2 | pTY60-fliG | 24 |
| pNT2 | pTY60-gfp-pomB, pomA | This study |
a
Tpr, trimethoprim resistant; Smr, streptomycin resistant; Rifr, rifampin resistant; Kmr, kanamycin resistant; Cmr, chloramphenicol resistant; Pof+, normal polar flagellar formation; Laf−, defective in lateral flagellar formation; Pom+, polar flagellar motility; Plac, lac promoter; PBAD, arabinose promoter.