FIG 5.

Three-dimensional reconstructions of biofilms formed by wild-type A. actinomycetemcomitans 652 (A) and isogenic ΔihfA (B) and ΔihfB (C) mutants. Mutations were complemented by a plasmid-borne copy of ihfA or ihfB in plasmids pATE92 and pATE93 (D and E), respectively. Biofilms were cultured in open flow cells as described in Materials and Methods and were visualized by confocal laser scanning microscopy. Image stacks were assembled and analyzed using Volocity image analysis software. The yellow and blue scale bars in the x-y, x-z, and y-z sections represent 50 μm and 10 μm, respectively.